生物影像分析在細胞生物學的應用
Applications of Image Analysis in Cell Biology
學期
106-1
學分
3
學分
當期課號
A510
永久課號
A1409
開課單位
生命科學系暨基因體科學研究所
授課教師
朱業修、蔡金吾、林崇智
校區
陽明
類別
選修
上課時間表
| 節 | 週二 |
|---|---|
6 14:20–15:10 | 生物影像分析在細胞生物學的應用 YL735 3 節連堂 |
7 15:30–16:20 | |
8 16:30–17:20 |
* 根據陽明交大上課時間表所列
週次計畫
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction |
| 第 2 週 | Fluorescence microscopy (I) 1. Fluorescence basic 2. Fluorescence probes 3. Fluorescence optics 4. Epi-microscopy |
| 第 3 週 | Fluorescence microscopy (II) 1. Confocal fluorescence microscopy 2. TIRFM 3. TIRFM for exocytosis and endocytosis |
| 第 4 週 | Basic light microscopy 1. Introduction 2. Light and optics 3. Phase contrast 4. DIC 5. Darkfield |
| 第 5 週 | Biological applications of fluorescence microscopy 1. Ratio imaging for [Ca2+]i 2. Ratio imaging for pH 3. FRAP/FLIP |
| 第 6 週 | Advanced fluorescence microscopy (I) FRET/Fluorescence lifetime imaging |
| 第 7 週 | Advanced fluorescence microscopy (II) FCS for molecular dynamics |
| 第 8 週 | Advanced fluorescence microscopy (III) Nonlinear and other light microscopy |
| 第 9 週 | Lab (I) Light microscopy(pm1:30-4:30) |
| 第 10 週 | Lab (II) Confocal microcopy(pm1:30-4:30) |
| 第 11 週 | Lab (III) TIRFM and Spining-disc confocal microcopy (pm1:30-4:30) |
| 第 12 週 | Lab (IV) Intravital Imaging lab (pm1:30-4:30) |
| 第 13 週 | Intravital Imaging |
| 第 14 週 | Superresolution fluorescence microscopy Electron microscopy and scanning probe microscopy |
| 第 15 週 | Digital imaging, image processing, quantification and feature extraction |
| 第 16 週 | Lab (V) Measurement of mitochondria morphology and dynamics of secretory vesicles |
| 第 17 週 | Lab (VI) Microscopy-based data mining: ImageJ, CellProfiler and Weka |
| 第 18 週 | Modeling cellular events and signal transduction by morphological features (許昭萍) |