高等生物技術導論
Advanced Biological Techniques
學期
106-1
學分
2
學分
當期課號
B156
永久課號
A3692
開課單位
口腔生物研究所
授課教師
黎萬君、陳恆理、曾才郁、黃何雄、洪善鈴、夏堪臺、張國威、羅正汎
校區
陽明
類別
必修
上課時間表
| 節 | 週四 |
|---|---|
1 08:00–08:50 | 高等生物技術導論 YD302 2 節連堂 |
2 09:00–09:50 |
* 根據陽明交大上課時間表所列
週次計畫
| 週次 | 主題 |
|---|---|
| 第 1 週 | Cell culture and bacterial culture techniques |
| 第 2 週 | Purification of plasmid DNA and genomic DNA |
| 第 3 週 | DNA fragment purification, agarose and polyacrylamide gel electrophoresisBasic concepts of biomaterials surface science |
| 第 4 週 | Basic concepts of biomaterials surface scienceDNA fragment purification, agarose and polyacrylamide gel electrophoresis |
| 第 5 週 | Restriction enzyme digestion and enzymatic manipulation of DNAPolymerase chain reaction, and Southern, Northern and Western blotting techniques |
| 第 6 週 | Polymerase chain reaction, and Southern, Northern and Western blotting techniquesReal-time PCR |
| 第 7 週 | Real-time PCRRestriction enzyme digestion and enzymatic manipulation of DNA |
| 第 8 週 | Introduction of DNA into bacteria and mammalian cells |
| 第 9 週 | 期中考 |
| 第 10 週 | Transgenic and gene knock-out mice |
| 第 11 週 | Introduction to gene therapy |
| 第 12 週 | Adenoviral and retroviral DNA delivery (第一節: 黎萬君老師) The genome editing: CRISPR (第二節: 曾才郁老師)Adenoviral and retroviral DNA delivery |
| 第 13 週 | RNA interference |
| 第 14 週 | Genomic and proteomic studyGenomic and proteomic study; Immunoassay and immunoprecipitation |
| 第 15 週 | Immunoassay (ELISA; immunoperoxidase assay) and immunoprecipitationIn situ hybridization, immunohistochemistry, LCM and image analysis |
| 第 16 週 | In situ hybridization, immunohistochemistry, LCM and image analysis開國紀念日 |
| 第 17 週 | Fluorescence-activated cell sorter (FACS) and confocal microscopy |
| 第 18 週 | 期末考 |