顯微鏡在細胞學之應用
Cell Biology Application of Microscopy
| 節 | 週二 |
|---|---|
3 10:10–11:00 | 顯微鏡在細胞學之應用 BC208 2 節連堂 |
4 11:10–12:00 |
* 根據陽明交大上課時間表所列
Microscopy has become one of the most utilized tool in the biology field. It is essential for modern day biologists to learn the basics of microscopy and how it should be properly used in studying biological events. This course will cover most techniques used in a cell biology lab ranging from contrast-based illumination to specialized fluorescence microscopy. This class will also discuss the potential problem of scientific misconducts related to image manipulation. 顯微技術已成為研究生物學最重要的工具之一。擁有基礎顯微常識及了解如何正確的使用顯微技術是現今從事生物學研究人員所不可或缺的知識。本課程將教授現今細胞生物實驗室所使用之顯微技術,涵蓋範圍從基本對比顯影技術至特殊螢光顯微技術。本課程並會討論與影像處理有關的科學倫理問題。
general biology, physics, and one semester of cell biology 普通生物學、物理學以及一學期的細胞生物學
1. Optical Microscopy Primer (http://micro.magnet.fsu.edu/primer/) 2. Zeiss Microscopy Campus (http://zeiss-campus.magnet.fsu.edu/index.html) 3. iBiology Microscopy Course (http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-course.html)
In-class quiz and discussion, midterm exam, and final exam 課堂小考和討論、期中及期末考
- Entire course 總課程
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction |
| 第 2 週 | Light, color, and resolution |
| 第 3 週 | Fundamental of light microscopy |
| 第 4 週 | Optical aberrations and objective lens |
| 第 5 週 | Conjugate planes and Kohler illumination |
| 第 6 週 | Cell culture and live cell imaging |
| 第 7 週 | Contrast-based imaging methods I |
| 第 8 週 | Contrast-based imaging methods II |
| 第 9 週 | Midterm exam |
| 第 10 週 | Digital Camera |
| 第 11 週 | Image processing and manipulation |
| 第 12 週 | Fluorescence microscopy |
| 第 13 週 | Confocal fluorescence microscopy |
| 第 14 週 | Multi-photon fluorescence microscopy |
| 第 15 週 | Advanced fluorescence microscopy techniques and nanoscopy (super-resolution microscopy) |
| 第 16 週 | Hands-on demo |
| 第 17 週 | Final exam |
PDF documents will be provided by the instructor 老師會提供上課講義
- 地點
- Bo-Ai campus, Zhu-Ming building, room 111 博愛校區竹銘館111室
- 時間
- Monday 16:00 - 18:00 星期一 16:00 - 18:00
- 聯絡方式
- campus phone extension 56968 校內分機 56968