生殖生物學實驗方法學
Experimental Methodology in Reproductive Biology
學期
106-2
學分
3
學分
當期課號
B136
永久課號
A1598
開課單位
生理學研究所
授課教師
黃娟娟
校區
陽明
類別
選修
上課時間表
| 節 | 週二 |
|---|---|
2 09:00–09:50 | 生殖生物學實驗方法學 YR105 3 節連堂 |
3 10:10–11:00 | |
4 11:10–12:00 |
* 根據陽明交大上課時間表所列
週次計畫
| 週次 | 主題 |
|---|---|
| 第 2 週 | 2/1 Lecture 1: Introduction – ovarian function & research background Laboratory Experiment: (1) Animal handling & Hormone injection & (2) preparation of culture wares |
| 第 3 週 | 2/2 Lecture 2: Methodology of cell culture Laboratory Experiment: Preparation of reagents & Coating culture plateswares / reagents |
| 第 4 週 | 2/3 Laboratory Experiment: Isolation of rat ovarian cells & Culture |
| 第 5 週 | 2/4 Laboratory Experiment: Change medium & Establishment of cell number standard curve 2/5 Laboratory Experiment: Treatment of cells & Preparation for sample harvest needs |
| 第 6 週 | 2/6 Lecture 3: Principle & application of gel electrophoresis 2/12 Laboratory Experiment: Treatment of cells & Preparation for sample harvest needs |
| 第 7 週 | 2/7 Laboratory Experiment: (1) Harvest of samples & Cell number determination assay, (2) Immunoblotting analysis (day1) |
| 第 8 週 | 2/8 Laboratory Experiment: Protein assay & Immunoblotting analysis (day2) |
| 第 9 週 | 2/9 Laboratory Experiment: Immunoblotting analysis (day 3) |
| 第 10 週 | 2/12 Lecture 4: Methodology of cell proliferation & apoptosis Laboratory Experiment: Zymography analysis (day 1) |
| 第 11 週 | 2/13 Laboratory Experiment: Zymography analysis (day 2) 2/14 Laboratory Experiment: Zymography analysis (day 3) |
| 第 12 週 | 2/23 Laboratory Experiment: Densitometric analysis-demonstration |
| 第 13 週 | 2/27 Lecture 5: Principle of enzyme immunoassays & nucleic analyses |
| 第 14 週 | 3/5 Laboratory Experiment: EIA- Establishment of standard curve (day 1) 3/6 Laboratory Experiment: EIA- Establishment of standard curve (day 2) & EIA- sample analysis (day 1) |
| 第 15 週 | 3/7 aboratory Experiment: EIA- sample analysis (day 2) |
| 第 16 週 | 3/13 Laboratory Experiment: Densitometric analysis of samples (1) |
| 第 17 週 | 3/20 Laboratory Experiment: Densitometric analysis of samples (2) |
| 第 18 週 | 3/27 Lecture 6: Data analysis & Discussion |