光學生物影像技術
Optical Bio-imaging
| 節 | 週四 |
|---|---|
2 09:00–09:50 | 光學生物影像技術 BI308 3 節連堂 |
3 10:10–11:00 | |
4 11:10–12:00 |
* 根據陽明交大上課時間表所列
介紹現有光學生物影像技術 目標: (1) To provide fundamental concepts in optics, laser, microscopy, linear and nonlinear molecular spectroscopy. (2) To help students develop physical understanding about the underlying principles of various emerging optical imaging techniques. (3) To motivate individuals into this exciting field to make innovation
基礎物理、基礎化學
Lecture/ PowerPoint slides: PowerPoint slides will be uploaded to the course website one week before the corresponding lectures
學期作業:佔總學期30分(約4~5份) 考試狀況:除了作業之外,其餘均為報告成績 評量方法 Home Work:30%; Presentation(case study):40%; Term Paper:30% ‧For Presentation, topic of specific case study will be given to each student in the middle of semester. Students will have at least two weeks preparation time before their individual presentation ‧For term paper, there are two options): Option A: Original proposal (less than 3 pages) of a new photon-based method for biological systems. A major modification on an existing method can be also considered. Option B: Review paper (10~15 pages) on the principles of a particular optical imaging method and its applications to biological studies. More than 30 references are required.
- Spectroscopy
- Microscopy
- Optical probes
- Super-resolution
- Applications
- Class discussion/ presentation
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction; Optics & light |
| 第 2 週 | Linear molecular spectroscopy |
| 第 3 週 | Fluorescence spectroscopy I |
| 第 4 週 | Fluorescence spectroscopy II |
| 第 5 週 | Nonlinear molecular spectroscopy |
| 第 6 週 | Basic light microscopy; Confocal microscopy, Total internal reflection; |
| 第 7 週 | National Holiday |
| 第 8 週 | Sheet illumination Multi-photon fluorescence imaging |
| 第 9 週 | Imaging probe development |
| 第 10 週 | Single-molecule biophysics |
| 第 11 週 | Super resolution imaging |
| 第 12 週 | Label-free chemical imaging |
| 第 13 週 | Emerging technology |
| 第 14 週 | More applications |
| 第 15 週 | More applications |
| 第 16 週 | Student presentations I |
| 第 17 週 | Student presentations II |
| 第 18 週 | Term paper hand in and discussions |
以下均為參考書目: ‧ P. N. Prasad, Introduction to Biophotonics, 1st Ed., New York : Wiley, 2003 ‧ J. R. Lakowicz, Principles of fluorescence spectroscopy, 3rd Ed., New York : Springer, 2006 ‧ J. Pawley (ed), Handbook of biological confocal microscopy, 2nd Ed., New York : Springer, 1995 ‧ R. W. Boyd, Nonlinear optics 3rd Ed., Boston: Academic Press, 2008 ‧ B. R. Masters and P. So, Handbook of biomedical nonlinear optical microscopy, 1st Ed., New York: Oxford University Press, 2008 ‧Rafael Yuste (ed.), Imaging: a laboratory manual, 1st Ed., New York: Cold Spring Harbor Laboratory, 2010.
- 地點
- 博愛校區 實驗一館111室
- 時間
- 週三 1:00~3:00
- 聯絡方式
- yatingkao @nctu.edu.tw