酵母菌之合成生物學實驗設計與探討
The implications of synthetic biology in yeast
學期
108-1
學分
3
學分
當期課號
A535
永久課號
B1404
開課單位
生命科學系暨基因體科學研究所
授課教師
陳瀅州
類別
選修
上課時間表
| 節 | 週二 |
|---|---|
2 09:00–09:50 | 酵母菌之合成生物學實驗設計與探討 3 節連堂 |
3 10:10–11:00 | |
4 11:10–12:00 |
* 根據陽明交大上課時間表所列
週次計畫
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction of the course – What is synthetic biology? |
| 第 2 週 | Yeast genetics – Getting started with yeast |
| 第 3 週 | Cell cycle of yeast – Culture and growth curve |
| 第 4 週 | Growth and manipulation of yeast – Genotype and phenotype |
| 第 5 週 | Growth and manipulation of yeast – Auxotroph and its implication in yeast genetics |
| 第 6 週 | Characterization of yeast mutants – Genetic complementation |
| 第 7 週 | Assay of yeast mating-type switches |
| 第 8 週 | Yeast meiosis and sporulation |
| 第 9 週 | Tetrad dissection and analysis |
| 第 10 週 | Mapping of yeast chromosomes |
| 第 11 週 | Gene linkage using meiotic mapping |
| 第 12 週 | Analysis of chromosome segregation in yeast |
| 第 13 週 | Epistasis analysis |
| 第 14 週 | Suppressor mutation – ade2 suppressor screening |
| 第 15 週 | Gene and gene interactions – synthetic lethality |
| 第 16 週 | Modern techniques for recombinant DNA assembly |
| 第 17 週 | Engineered and synthetic yeast – control and reprogram biological circuits |
| 第 18 週 | Final discussion and check-out |