生物影像分析在細胞生物學的應用
Applications of Image Analysis in Cell Biology
學期
108-1
學分
3
學分
當期課號
A548
永久課號
A1409
開課單位
生命科學系暨基因體科學研究所
授課教師
朱業修、林崇智、賈世璿
校區
陽明
類別
選修
上課時間表
| 節 | 週四 |
|---|---|
2 09:00–09:50 | 生物影像分析在細胞生物學的應用 YL735 3 節連堂 |
3 10:10–11:00 | |
4 11:10–12:00 |
* 根據陽明交大上課時間表所列
週次計畫
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction |
| 第 2 週 | Fluorescence microscopy (I) 1. Fluorescence basic 2. Fluorescence probes 3. Fluorescence optics 4. Epi-microscopy |
| 第 3 週 | Basic light microscopy 1. Introduction 2. Light and optics 3. Phase contrast 4. DIC 5. Darkfield |
| 第 4 週 | Fluorescence microscopy (II) 1. Confocal fluorescence microscopy 2. TIRFM 3. TIRFM for exocytosis and endocytosis |
| 第 5 週 | Biological applications of fluorescence microscopy 1. Ratio imaging for [Ca2+]i 2. Ratio imaging for pH 3. FRAP/FLIP |
| 第 6 週 | Intravital microscopy |
| 第 7 週 | Advanced fluorescence microscopy (I) FRET/Fluorescence lifetime imaging |
| 第 8 週 | Advanced fluorescence microscopy (II) FCS for molecular dynamics |
| 第 9 週 | Advanced fluorescence microscopy (III) Nonlinear and other light microscopy |
| 第 10 週 | Superresolution microscopy |
| 第 11 週 | Electron microscopy, Scanning probe microscopy |
| 第 12 週 | Lab (I) Basic optics alignment, phase contrast and DIC micorscopy-lab (am9:00-12:00) |
| 第 13 週 | Lab (II) Confocal microcopy-lab (am9:00-12:00) |
| 第 14 週 | Lab (III) TIRFM and Spining-disc confocal microcopy (am9:00-12:00) |
| 第 15 週 | Digital imaging, image processing and feature extraction |
| 第 16 週 | Microscopy-based data mining: ImageJ, CellProfiler and Weka |
| 第 17 週 | Lab (IV) Numerical morphological analysis of 3D mitochondria morphology by microP3D |
| 第 18 週 | Lab (VI) Profiling drug effects on mitochondrial and chromosomal partition by high content analysis |