基因工程實作
Genetic Engineering Lab
學期
108-2
學分
1
學分
當期課號
A558
永久課號
B1195
開課單位
生物醫學影像暨放射科學系
授課教師
莊惠燕
類別
選修
上課時間表
| 節 | 週一 | 週四 |
|---|---|---|
1 08:00–08:50 | 基因工程實作 | 基因工程實作 |
* 根據陽明交大上課時間表所列
週次計畫
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction of laboratory and how to record your experiments |
| 第 1 週 | Introduction of laboratory and how to record your experiments |
| 第 2 週 | Lab Safety |
| 第 2 週 | Lab Safety |
| 第 3 週 | Introduction of DNA digestion and DNA electrophoresis |
| 第 3 週 | Introduction of DNA digestion and DNA electrophoresis |
| 第 4 週 | DNA digestion and DNA electrophoresis |
| 第 4 週 | DNA digestion and DNA electrophoresis |
| 第 5 週 | Competent cell preparation and transformation |
| 第 5 週 | 清明連假 |
| 第 6 週 | 校際活動周 |
| 第 6 週 | Competent cell preparation and transformation |
| 第 7 週 | How to read a scientific paper and how to prepare your presentation |
| 第 7 週 | How to read a scientific paper and how to prepare your presentation |
| 第 8 週 | Introduction of DNA detection: PCR and primer design |
| 第 8 週 | DNA amplification (PCR) and gel extraction |
| 第 9 週 | DNA amplification (PCR) and gel extraction |
| 第 9 週 | Ligation and transformation |
| 第 10 週 | Ligation and transformation |
| 第 10 週 | Introduction of DNA extraction and Southern blotting |
| 第 11 週 | Detection of specific DNA sequence- DNA extraction and PCR |
| 第 11 週 | Detection of specific DNA sequence- DNA extraction and PCR |
| 第 12 週 | Aseptic techniques for cell culture |
| 第 12 週 | Aseptic techniques for cell culture |
| 第 13 週 | Introduction of gene transfer, stable clone establishment and checkup the transfected cells |
| 第 13 週 | Introduction of gene transfer, stable clone establishment and checkup the transfected cells |
| 第 14 週 | Protein samples and reagent preparation for Western blotting |
| 第 14 週 | Protein samples and reagent preparation for Western blotting |
| 第 15 週 | Western blotting |
| 第 15 週 | Western blotting |
| 第 16 週 | Current topics of genetic engineering |
| 第 16 週 | Current topics of genetic engineering |
| 第 17 週 | Final Presentation (進行網路課程)Final Presentation |
| 第 17 週 | Final Presentation (進行網路課程)Final Presentation |
| 第 18 週 | Final Presentation (進行網路課程)Final Presentation |
| 第 18 週 | Final Presentation (進行網路課程)Final Presentation |