生物序列分析與高通量技術
Sequencing technology and high-throughput data analysis
| 節 | 週一 |
|---|---|
5 13:20–14:10 | 生物序列分析與高通量技術 BI305 3 節連堂 |
6 14:20–15:10 | |
7 15:30–16:20 |
* 根據陽明交大上課時間表所列
課程概述與目標: 本課程主要介紹次世代定序資料在目前生命科學研究上的應用與分析處理方法, 包括常見的全基因體定序、外顯組定序、轉錄體定序、微生物體定序等,課程中除了介紹原理外,亦將以課堂討論的方式介紹該領域最新的研究成果範例。 此課程亦將包含實作部分, 目前有不少跨平台的分析次世代資料的工具也支援在 windows 上運行,但是大部分工具還是以 Unix 為主要作業系統,因此學期前段會以數堂課時間讓學員熟悉 Unix 的操作介面並介紹 R 語言。 (This class introduces the history of sequencing technologies and the applications of Next Generation Sequencing (NGS) and provides some examples of the basic analysis. NGS strategies including whole genome sequencing, whole exome sequencing, RNA sequencing and metagenomics will be covered. Given that most of the NGS tools are Linux based we will first give students a taste of the Linux system in the beginning of the course. Linux system and the scripting programming language R will be used in most of the examples.)
基本分子生物學概念 (basic knowledge in molecular biological)
1. Handouts 2. Board writing 3. Panel discussion
評量方法 : Midterm (25%); Home work (25%); oral presentation (Linux, 15%); (in class) discussion & paper reading (10%); oral presentation (25%)
- Introduction of next generation sequencing (NGS)
- Introduction of Unix
- Introduction of R
- Whole genome sequencing
- Target sequencing
- Transcriptome profiling & functional analysis
- Small RNA Sequencing
- Bacterial genome assembly
- Introduction
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction |
| 第 2 週 | Introduction of Linux (I) |
| 第 3 週 | Introduction of Linux (II) |
| 第 4 週 | Introduction of Next Generation Sequencing (NGS) |
| 第 5 週 | Whole genome sequencing |
| 第 6 週 | 生技公司參觀 |
| 第 7 週 | Target sequencing (Whole exome sequencing & disease panels) |
| 第 8 週 | Midterm |
| 第 9 週 | Introduction of R |
| 第 10 週 | Transcriptome profiling & functional analysis |
| 第 11 週 | Transcriptome profiling & functional analysis (II) |
| 第 12 週 | 期末報告預演 (I) |
| 第 13 週 | Linux複習考+Small RNA Sequencing |
| 第 14 週 | 期末報告預演 (II) |
| 第 15 週 | Bacterial genome assembly (NGS & 3rd generation sequencing) |
| 第 16 週 | NGS for non-model organism |
- 地點
- 賢齊樓 420室
- 時間
- 周五 9:00-11:00
- 聯絡方式
- 03-5712121 ext. 59734