分子生物技術
Techniques of Molecular Biology
| 節 | 週五 |
|---|---|
5 13:20–14:10 | 分子生物技術 YR105 2 節連堂 |
6 14:20–15:10 |
* 根據陽明交大上課時間表所列
1. Nucleic acid isolation, electrophoresis, nucleic acid hybridization, Southern & Northern blotting 2. Restriction endonuclease and DNA cloning 3. PCR and DNA sequencing 4. Gene delivery techniques: Transformation, Transfection & Infection 5. Methods in reconstructing a phylogenetic tree using molecular data 6. Genomic approaches & bioinformatics 7. Genotyping 8. Protein overexpression, purification, and detection 9. Protein-protein interaction, nucleic acid-protein interactions and immunoprecipitation & ChIP 10. Proteomics approaches 11. Radiobioassay 12. Fluorescent microscopy and image 13. Flow cytometry 14. Metabolomics 15. Electrophysiological recording techniques
1. 瞭解分子生物學技術之原理與操作 2. 瞭解分子生物學技術在轉譯醫學研究上的應用 3. 瞭解細胞生物學技術之原理與操作 4. 瞭解細胞生物學技術在轉譯醫學研究上的應用
1. 課中討論 2. 分組討論 3. 書面報告
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction(https://meet.google.com/wzh-hyux-zov) PCR and DNA sequencingPCR and DNA sequencing |
| 第 2 週 | Restriction endonuclease and DNA cloning |
| 第 3 週 | Nucleic acid isolation, electrophoresis, nucleic acid hybridization, Southern & Northern blotting |
| 第 4 週 | Gene delivery techniques: Transformation, transfection & infection |
| 第 5 週 | Genomic approaches |
| 第 6 週 | Real-time quantitative PCR |
| 第 7 週 | Methods in reconstructing a phylogenetic tree using molecular data |
| 第 8 週 | Mass spectrometry |
| 第 9 週 | Electrophysiological recording techniques |
| 第 10 週 | MetabolomicsProtein-protein interaction, nucleic acid-protein interactions and immunoprecipitation & ChIP |
| 第 11 週 | Proteomics approaches |
| 第 12 週 | Fluorescence imaging |
| 第 13 週 | Protein overexpression, purification, and detection |
| 第 14 週 | Protein-protein interaction, nucleic acid-protein interactions and immunoprecipitation & ChIP Metabolomics |
| 第 15 週 | Flow cytometry |
| 第 16 週 | 開國紀念日調整放假 |
| 第 17 週 | 自主學習(課後報告) |
| 第 18 週 | 自主學習(課後報告) |
1. Molecular Cloning (4th ed., Michael R. Green and Joseph Sambrook) 2. Short Protocols in Cell Biology (1st ed., Juan S. Bonifacino, Mary Dasso, Joe B. Harford, Jennifer Lippincott-Schwartz, and Kenneth M. Yamada)