小鼠遺傳、發育與表現型分析
Mouse Genetics, Development and Phenotypes
| 節 | 週二 |
|---|---|
5 13:20–14:10 | 小鼠遺傳、發育與表現型分析 YC501 2 節連堂 |
6 14:20–15:10 |
* 根據陽明交大上課時間表所列
1. 基因轉殖/剔除小鼠建立的基本概念,表現型分析常用的研究方法 2. 早期發育、器官形成與胚胎致死 3. 幹細胞及細胞譜系決定 4. 上皮細胞恆定與癌症形成 5. 免疫系統的發育與成熟 6. 行為表現型分析 1. Transgenic and knockout mice: Principle, application, and techniques for visualizing gene products 2. Mouse early development, organogenesis and embryonic lethality 3. Stem cells and cell lineage determination 4. Epithelial homeostasis and mouse cancer genetics 5. Development and maturation of immune system 6. Behavioral phenotyping
基因轉殖/剔除小鼠是研究基因功能及疾病機制的動物模式,亦可做為藥物研發的重要工具。本課程將介紹基因轉殖/剔除小鼠建立的基本概念,解說表現型分析常用的研究方法。透過基因突變鼠造成的表現型改變來闡明各種基因的作用機制在細胞、組織和生命個體中所扮演的生理功能,提升文獻閱讀能力,在面對日新月異的醫學科學研究相關內容能有更深刻的了解。Transgenic/knockout mice are important tools for elucidating gene function and disease mechanisms in vivo and proposing new drug and treatment strategies. This course provides basic concept of mouse genetics, development, and phenotyping. At the end of this course, students will gain knowledge of different mouse models and their normal functions and/or roles in diseases as well as their applications in biomedical research.
作業或考試Homework or examination
| 週次 | 主題 |
|---|---|
| 第 1 週 | Transgenics: Principle, application, and techniques for visualizing gene products |
| 第 2 週 | Conditional gene manipulation |
| 第 3 週 | Embryonic lethality during organogenesis |
| 第 4 週 | Mouse early development and axis patterning |
| 第 5 週 | Stem cells and cell lineage determination |
| 第 6 週 | Getting around an early lethal phenotype |
| 第 7 週 | Epithelial homeostasis and diseases |
| 第 8 週 | 民族掃墓節 (放假) |
| 第 9 週 | Mouse cancer genetics- I |
| 第 10 週 | Mouse cancer genetics- II |
| 第 11 週 | Development and maturation of immune system |
| 第 12 週 | Immunological analysis on the mutant mice-I |
| 第 13 週 | Immunological analysis on the mutant mice-II |
| 第 14 週 | Behavioral phenotyping-I |
| 第 15 週 | Behavioral phenotyping-II |
| 第 16 週 | Behavioral phenotyping-III |
| 第 17 週 | Mouse genetics (彈性補充教學) |
| 第 18 週 | Mouse phenotyping (彈性補充教學) |