高等生物物理技術

Advanced Biophysical Technology

學期

112-1

學分

3 學分

當期課號

430017

永久課號

BTBT30184

開課單位

生物科技學系

授課教師

張家靖

校區

博愛

類別

選修

上課時間表

* 根據陽明交大上課時間表所列

概述

從生物物理實驗技術入門,進而瞭解其所蘊藏的物理與生化原理。

先修科目

物理,化學,生物

評分方式

Midterm 30% Containing: test and experimental operation. Presentation and discuss 30% Final test 40%

週次計畫

週次	主題
第 1 週	Introduction a. Factor affecting analyte structure and stability
第 2 週	b. Buffer system*c. Quantitation
第 3 週	Spectroscopic Techniques a. Principle b. Electromagnetic (EM) spectrum c. UV/Vis absorption spectroscopy* d. Fluorescence spectroscopy
第 4 週	e. Circular dichroism (CD)* f. Infrared spectroscopy* g. Raman spectroscopy* h. NMR spectroscopy i. Electron spin resonance (ESR) spectroscopy
第 5 週	Hydrodynamic Methods a. Semi-permeable membrane* b. Viscosity* c. Thermal equilibrium dialysis*
第 6 週	d. Sedimentation* e. Micro-cone* f. Flow cytometry g. Dynamic light scattering h. Static light scattering
第 7 週	Electrophoresis a. Principle b. DNA gel electrophoresis* c. RNA gel electrophoresis
第 8 週	d. Protein electrophoresis* e. Two-dimensional electrophoresis
第 9 週	Chromatography a. Principle b. Equipment* c. Modes of chromatography*
第 10 週	d. HPLCe. Perfusion HPLC
第 11 週	Mass spectrometry a. Principle b. MS/MS c. LC/MS d. GC/MS e. Eletrophoresis/MS
第 12 週	Biocalorimetry a. Principle b. Isothermal titration calorimetry c. Differential scanning calorimetry*
第 13 週	Three-dimensional structure determination of macromoleculesa. Structure determination by NMRb. X-ray diffraction by crystalc. Electron diffraction
第 14 週	Computational Biology a. Molecular modeling b. MC/MD simulation
第 15 週	Specific topics presentation and discussion
第 16 週	Specific topics presentation and discussion
第 17 週	彈性課程周
第 18 週	彈性課程周

教科書

Physical Biochemistry: Principle and Applications.2nd edition Author: David Sheehan Publisher: John Wiley and sons, LTD (2009)

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