微生物基因工程
Microbial Genetic Engineering
| 節 | 週五 |
|---|---|
5 13:20–14:10 | 微生物基因工程 BI302 3 節連堂 |
6 14:20–15:10 | |
7 15:30–16:20 |
* 根據陽明交大上課時間表所列
Class Description: “Microbial Genetic Engineering " is a comprehensive, hands-on course designed to guide students through the fundamental techniques employed in the rapidly evolving fields of Genetic Engineering. The course covers a range of topics including plasmid construction, gene expression manipulation, bacterial host genomic modifications, enzyme bioprospecting, enzyme assay and pathway assay, protein engineering and direct evolution, and an introduction to the latest sequencing technologies. Through real-world examples, students will learn how to design, conduct, and interpret experimental results, and gain a deeper understanding of the practical aspects of these technologies. Purpose of the Class: The purpose of this course is to equip students with the practical skills and knowledge in Genetic Engineering, which are essential for contributing to research and development in the fields of Metabolic Engineering and Synthetic Biology. It aims to give students a solid foundation of key techniques, encourage critical thinking, promote innovative experimental design, and foster an understanding of the practical applications of these concepts in various applications, including biotechnology, biofuel and biochemical productions, and pharmaceuticals.
Molecular biology (I), Biochemistry (I) & (II), and/or Microbiology, are recommended.
Final exam : 30 % Assignments: 30 % Presentation: 25 % Class participation: 15 %
| 週次 | 主題 |
|---|---|
| 第 1 週 | Course introduction and overview |
| 第 2 週 | Introduction of expression systems: promoter, RBS and plasmid origins |
| 第 3 週 | Mid-Autumn Festival, no class |
| 第 4 週 | Steps by steps for plasmid constructions (with introduction of software and online tools) |
| 第 5 週 | Case study: Promoter prospecting in Geobacillus |
| 第 6 週 | Case study: Enzyme bioprospecting (using Kivd as an example) (with Assignments) |
| 第 7 週 | Case study: Enzyme assay & Pathway assay (with Assignments) (Using Kivd, Adh as example for enzyme assay; using rGPS-MCG pathway as an example for pathway assay) |
| 第 8 週 | Protein engineering & Direct evolution |
| 第 9 週 | Case study: Protein engineering & Direct Evolution (using Kivd and Mdh as examples) |
| 第 10 週 | Bacterial host genomic modifications |
| 第 11 週 | Case study: bacterial host genomic modifications – gene deletion (with Assignments) (Using ptsG deletion for lambda-Red system, lsrF deletion for CRISPR system) |
| 第 12 週 | Case study: Bacterial host genomic modifications – gene integration (using tal integration CRISPR and Transposon-encoded CRISPR–Cas systems) |
| 第 13 週 | Sanger sequence applications |
| 第 14 週 | NGS & Nanopore applications |
| 第 15 週 | Literature presentation and discussion |
| 第 16 週 | Final exam week |
Primary material: Lecture notes
- 地點
- Bio-ICT Room 519
- 時間
- By appointment
- 聯絡方式
- Email: linp@nycu.edu.tw