離子通道與膜電生理概論
Introduction of ion channels and membrane electrophysiology
| 節 | 週四 |
|---|---|
N 12:20–13:10 | 離子通道與膜電生理概論 YR102 |
* 根據陽明交大上課時間表所列
本課程的內容包含離子通道電生理及其調控機制,細胞膜電位及離子通透原理。同時也會介紹離子通道的結構,如何篩選特定離子通過,離子通道在神經及心臟系統的功能及相關疾病。This course covers selected topics of ion channel biophysics for students with a major interest in membrane physiology. Topics to be covered include membrane potential, voltage-dependent channel gating, ion channel blockers, ion permeation through channel pores, structure/function relationships of ion channels and cell biology of ion channels. The role of ion channels in neurology and cardiology and relevant channelopathies will be introduced as well.
本課程的目標是瞭解細胞膜電生理學,離子通道功能及相關研究之方法學 The goal of this course is to understand membrane biophysics, gating of ion channels and recent advances in the methodology of ion channel related studies.
課程參與40%,第一次期中考20%,第二次期中考20%,期末考20% Attendance and in-class performance 40%,1st mid-term exam 20%, 2nd mid-term exam 20%, final exam 20%
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction of basic membrane physiology I: Diffusion, Chemical equilibrium, Protein Biochemistry, and Electricity Primer. |
| 第 2 週 | Introduction of basic membrane physiology II: Diffusion, Chemical equilibrium, Protein Biochemistry, and Electricity Primer. |
| 第 3 週 | Resting Membrane Potential I: Nernst Equation, Goldman-Hodgkin-Katz Equation |
| 第 4 週 | Resting Membrane Potential II: Nernst Equation, Goldman-Hodgkin-Katz Equation |
| 第 5 週 | First exam |
| 第 6 週 | Ion Permeations I: Long Pore Effect, Ion Binding and Saturation, Ion Selectivity, Multi-Ion Occupancy. |
| 第 7 週 | Ion Permeations II: Long Pore Effect, Ion Binding and Saturation, Ion Selectivity, Multi-Ion Occupancy. |
| 第 8 週 | Channel Blockers: Woodhull Model, Channel Structure and Function Implied from Blocker Actions |
| 第 9 週 | 2nd exam |
| 第 10 週 | Voltage-Dependent Mechanism: Voltage Dependence, Gating Charge, and Voltage Sensor |
| 第 11 週 | Methodology of studying ion channels I: basic concepts of patch clamp technique and its applications |
| 第 12 週 | Methodology of studying ion channels II: interpretation of results from patch clamp studies |
| 第 13 週 | Application of ion channel studies in neurology: introduction of channelopathy in neurology and pharmacological application |
| 第 14 週 | Application of ion channel studies in pulmonary: introduction of channelopathy in neurology and pharmacological application |
| 第 15 週 | Application of ion channel studies in cardiology: introduction of channelopathy in cariology and pharmacological application |
| 第 16 週 | Final exam |
| 第 17 週 | 補充教學 How to use mutagenesis to study a ion channel |
| 第 18 週 | 補充教學Application of ion channel studies:modern therapeutics related to ion channels |
Hille, B. (2001) Ion Channels of Excitable Membranes, 3rd Ed. Sinauer Associates, Inc. Sunderland, MA, USA.