生殖生物學實驗方法學
Experimental Methodology for Reproductive Biology
學期
113-1
學分
2
學分
當期課號
130012
永久課號
MDPY30018
開課單位
生理學研究所
授課教師
黃娟娟
校區
陽明
類別
選修
上課時間表
| 節 | 週六 |
|---|---|
1 08:00–08:50 | 生殖生物學實驗方法學 YR402 2 節連堂 |
2 09:00–09:50 |
* 根據陽明交大上課時間表所列
概述
學習基礎生物醫學研究相關方法學之原則及要點以利進行研究內分泌荷爾蒙分子 在細胞層次之作用機制與生理功能 To learn the principle of basic biological science research and methodologies for study the action mechanisms and biological functions of hormones/factors.
評分方式
實驗報告 70%; 討論 30% Experimental report 70%; Discussion 30%
課程大綱
- 講授課程 Lecture 1-8
- 實體實驗課 Laboratory Experiment
週次計畫
| 週次 | 主題 |
|---|---|
| 第 1 週 | 8/1 Lecture 1: Introduction of Ovarian Physiology and Q&A |
| 第 2 週 | 8/2 Lecture 2: Introduction of Research background and Q&A |
| 第 3 週 | 8/5 Lecture 3: Methodology of animal care, behavior & Cell culture |
| 第 4 週 | 8/6 Laboratory Experiment: Introduction of experimental animal facility and animal care Lecture 4: Methodology of cell viability and DNA content determinations |
| 第 5 週 | 8/7 Laboratory Experiment: Animal handling & Hormone injection |
| 第 6 週 | 8/8 Laboratory Experiment: Coating culture plates |
| 第 7 週 | 8/9 Laboratory Experiment: Isolation of rat ovarian cells & Culture |
| 第 8 週 | 8/10 Laboratory Experiment: Changing medium & Establishment of cell number standard curve |
| 第 9 週 | 8/11 Laboratory Experiment: Treatment of cells & Preparation for sample harvest needs |
| 第 10 週 | 8/13 Laboratory Experiment: Harvest of samples & Determination of cell number and protein content 8/15 Lecture 5: Data analysis & Discussion (I) |
| 第 11 週 | 8/19 Lecture 6: Principle and applications of gel electrophoresis |
| 第 12 週 | 8/20 Laboratory Experiment: Immunoblotting analysis-Day 1 |
| 第 13 週 | 8/21 Laboratory Experiment: Immunoblotting analysis-Day 2 |
| 第 14 週 | 8/23 Laboratory Experiment: Densitometric analysis of samples |
| 第 15 週 | 8/27 Lecture 7: Methodology of immunofluorescence |
| 第 16 週 | 8/28 Lecture 8: Data analysis & Discussion (II) |
教科書
國際科學期刊文獻以及相關之方法學工具書(參考講義所列文獻)