結構生物學(二)
Structural Biology II
| 節 | 週二 |
|---|---|
5 13:20–14:10 | 結構生物學(二) YL405 2 節連堂 |
6 14:20–15:10 |
* 根據陽明交大上課時間表所列
本課程內容規劃為四部份,依序如:(一)生物巨分子的電腦模擬:包生物資訊程式介紹及電腦輔助藥物設計的原理與應用。學習如何檢索相關結構生物學資料庫及生物分子系統模擬之應用;(二)高解相核磁共振光譜法:包括核磁共振在生物巨分子結構分析之原理與應用,學習如何讀譜分析結構和多維NMR在生物巨分子結構分析之應用;(三)無序蛋白質結構分析計算,光學光譜與溶液散射應用:包括原理及生物功能上的應用介紹。(四)X-光繞射與冷凍電子顯微鏡原理與應用:包括原理及分子結構分析之應用介紹。藉著這些方法與工具,來輔助鑑定生物巨分子之結構及探討其結構與功能之間的關係。The content of this course includes (1) Computer simulation of biomolecules: theoretical aspects of drug design, application of molecular modelling, and protein–Ligand interactions and molecular docking; (2) High resolution nuclear magnetic resonance spectroscopy: the principle and application of nuclear magnetic resonance, parameters of NMR and protein structure, and multi-dimensionality NMR in the structural analysis; (3) Computational approaches for intrinsically disordered proteins: optical spectroscopy and solution scattering; (4) X-ray diffraction and cryo-electron microscopy: principles and application. The application of these methods and tools is useful to identify the structures of biological macromolecules and further explore the relationship between the structure and function.
本課程規畫給生命科學、生物化學、藥學和生醫材料相關的學生及對結構生物學有興趣的研究學者。教學目標為學習生物分子模擬與藥物設計之原理與應用、高解相核磁共振光譜法在生物巨分子結構分析之原理與應用、光學光譜與溶液散射應用在無序蛋白質結構分析的應用和X-光繞射與冷凍電子顯微鏡原理與應用。藉著這些方法與工具,來輔助鑑定生物巨分子之結構及探討其結構與功能之間的關係。This course is designed for life science, biochemistry, pharmacy, and biomedical material students and research scientists. The course topics include basic principles and applications of Computer simulation of biomolecules, High resolution nuclear magnetic resonance spectroscopy, Computational approaches for intrinsically disordered proteins, and X-ray diffraction and cryo-electron microscopy. The application of these methods and tools is useful to identify the structures of biological macromolecules and further explore the relationship between the structure and function.
考試或書面報告exam or term paper
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction to computer-aided drug design (textbook 6) 計算機輔助藥物設計導論 |
| 第 2 週 | Theoretical aspects of drug design: quantum mechanics and molecular mechanics (textbook 4) 藥物設計的理論:量子力學和分子力學 |
| 第 3 週 | Application of molecular modelling (textbook 4)建立分子模型的應用 |
| 第 4 週 | Protein–Ligand Interactions and Molecular docking(textbook 1, chapter 8)分子對接:蛋白質與藥物分子作用 |
| 第 5 週 | Exam 1 |
| 第 6 週 | Principle of NMR (textbook 5)核磁共振光譜學基本原理 |
| 第 7 週 | Parameters of NMR and protein structure 核磁共振參數與蛋白質結構 |
| 第 8 週 | Multi-dimensional NMR spectroscopy 多維核磁共振光譜學 |
| 第 9 週 | Structure solving by NMR spectra 利用核磁共振光譜決定蛋白質結構 |
| 第 10 週 | Exam 2 |
| 第 11 週 | Computational approaches for intrinsically disordered proteins |
| 第 12 週 | Optical spectroscopy and solution scattering |
| 第 13 週 | X-ray diffraction |
| 第 14 週 | X-ray diffraction |
| 第 15 週 | Principle and application of cryo-EM |
| 第 16 週 | Exam 3 |
參考書: 1. Introduction to Proteins: Structure, Function, and Motion (2011) (Amit Kessel & Nir Ben-Tal) 2. Textbook of Structural Biology (2009) (Anders Liljas et al.) 3. Crystallization of Biological Macromolecules (1999) (Alexander McPherson) 4. Molecular modeling: Principles and Applications (Andrew R. Leach) 5. NMR of Macromolecules – A practical approach (G. C. Roberts) 6. Computer-Aided drug design: methods and applications (T. J. Perun and C. L. Propst)