光電生物物理技術
Optical and Electrical Techniques in Biophysics
| 節 | 週三 |
|---|---|
3 10:10–11:00 | 光電生物物理技術 BI301 2 節連堂 |
4 11:10–12:00 |
* 根據陽明交大上課時間表所列
課程概述與目標: 概論光電元件於生物物理實用技術,並介紹如何將這些技術實際運用於現代分子和細胞生物物理和生物化學研究◦內容包括:幾何光學、光學元件原理與應用、常見用於生物物理實驗之電子儀器與應用實例◦ This course will provide fundamental concepts on optical and electrical techniques in biophysics. Those techniques are general used to study the structure and function of biological macromolecules. The goal of this course is to help students to develop physical understanding about daily-used instruments in biophysical and biochemistry laboratory.
基礎物理、基礎化學 (General physics and chemistry)
Lecture/ PowerPoint slides
作業:3~5 homework sets (30% of the total final score) 作業繳交要求:可繳交紙本,亦可繳交電子檔並上傳於E3。選擇繳交電子檔,請掃描或拍照清楚,並整合為一個檔案,非壓縮檔。 考試:one take-home midterm and one take-home final exam ( 70% of the total final score)
- Light and Optics
- Electronics
- Detector and detections
- microscopy
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction; Light and Matters; Spectroscopy (Absorption) |
| 第 2 週 | Spectroscopy (Fluorescence) |
| 第 3 週 | Spectroscopy (IR and Raman) |
| 第 4 週 | Optical components I |
| 第 5 週 | Optical components II |
| 第 6 週 | Optical components III |
| 第 7 週 | Light source: incoherent light and lasers |
| 第 8 週 | Noise and response; Detector and detection |
| 第 9 週 | Electronic circuit; amplifier systems |
| 第 10 週 | Detector and detection; Electronic circuit |
| 第 11 週 | Oscilloscope; Function generator |
| 第 12 週 | Basic light microscopy, Confocal microscopy; Multi-photo fluorescent microscopy |
| 第 13 週 | Total internal reflection microscopy; Light-sheet illumination |
| 第 14 週 | Super-resolution microscopy |
| 第 15 週 | Penetration-depth improvement; CLARITY |
| 第 16 週 | Case studies |
| 第 17 週 | Q&A for final exam |
| 第 18 週 | Q&A for final exam |
以下均為參考書目: ‧ John H. Moore, Christopher C. Davis, Michael A Coplan and Sandra C. Greer, Building scientific apparatus, 4th Ed., Cambridge, 2009 ‧Eugene Hecht, Optics, 4th Ed., Addison-Wesley, 2011 ‧ Joseph R. Lakowicz, Principles of fluorescence spectroscopy, 3rd Ed., Springer, 2010 ‧ Frank Trager ed., Spring handbook of lasers and optics, 2007
- 地點
- 生物科技學系 賢齊舘424室(博愛校區)
- 時間
- 週三 8:00~10:00
- 聯絡方式
- yatingkao@nycu.edu.tw