離子通道分子物理學
Molecular Biophysics of Ion Channels
| 節 | 週二 | 週四 |
|---|---|---|
9 17:30–18:20 | 離子通道分子物理學 YR104 2 節連堂 | 離子通道分子物理學 YR104 2 節連堂 |
A 18:30–19:20 |
* 根據陽明交大上課時間表所列
課程概述與目標： 培養學生利用離子通道專業知識來增進自主學習以及邏輯推理能力: 這門課程專為有意從事學術研究的研究生以及高年級大學生設計。課程內容主要在于理解離子通道的分子結構和功能。本課程旨在培養定量分析和邏輯推理的能力，適合具備基礎數理分析以及生物化學知識的學生。本課程期望學生積極參與課堂討論，並學會批判性地閱讀原始論文。 1. 本課程旨在培養定量分析與邏輯推理的能力，以了解離子通道的結構與功能關係。 2. 學生將學習如何將基本的物理與化學原理應用於生物系統—細胞膜上的離子通道分子。 3. 通過閱讀原始論文和討論，學生將培養自學能力及批判性文獻閱讀技巧。 4. 學生將透過課程設計的實驗進行實作，熟悉重要的研究方法（如電壓鉗及膜片鉗電生理技術）。 Taking advantage of ion channel knowledge to enhance the ability of self-learning and logical reasoning: The course is designed for graduate students and advanced undergraduates who are interested in pursuing academic research. The course focuses on structures and functions of ion channels. It is aimed to cultivate students’ ability in quantitative analysis and logical reasoning. Students with basic quantitative analysis and biochemistry knowledge are encouraged to enroll. The course is aimed at training students to read original papers critically and to actively participate in course discussion. 1. This course aims to cultivate quantitative analysis and logical reasoning to understand the structure-functional relationship of ion channels. 2. Students will learn how to apply basic physical and chemical principles to a biological system, the ion channel molecule on the cell membrane. 3. Through original paper reading and discussion, students will develop skills of self-learning and critical literature reading. Students will be familiar to important research methods (such as voltage clamp and patch clamp electrophysiological techniques) through hands-on practices from experiments designed in the course.
1.學期作業、考試、評量 上台報告25％，書面報告25％，提問25％，課堂討論表現之觀察評量25％ 2.教學方法及教學相關配合事項（如助教、網站或圖書及資料庫等） 大堂課講授/實驗教學與操作/模擬實驗訓練
| 週次 | 主題 |
|---|---|
| 第 1 週 | (本週老師出國開會，故停課一次，原課程內容會協調調課)Course Introduction Diffusion & Chemical equilibrium (Learning HHSim computer program) |
| 第 1 週 | (本週老師出國開會，故停課一次，原課程內容會協調調課)Course Introduction Diffusion & Chemical equilibrium (Learning HHSim computer program) |
| 第 2 週 | Electricity Primer and Protein Structure. (Learning HHSim computer program) |
| 第 2 週 | Electricity Primer and Protein Structure. (Learning HHSim computer program) |
| 第 3 週 | Resting Membrane Potential (I): Nernst Equation (Learning HHSim computer program) |
| 第 3 週 | Resting Membrane Potential (I): Nernst Equation (Learning HHSim computer program) |
| 第 4 週 | Resting Membrane Potential (II): Goldman-Hodgkin-Katz Equation, paper discussion (Learning UCSF Chimera Program) |
| 第 4 週 | Resting Membrane Potential (II): Goldman-Hodgkin-Katz Equation, paper discussion (Learning UCSF Chimera Program) |
| 第 5 週 | Ion Permeations(I): Long Pore Effect, Ion Binding and Saturation, Ion Selectivity, Multi-Ion Occupancy. (Learning UCSF Chimera Program) |
| 第 5 週 | Ion Permeations(I): Long Pore Effect, Ion Binding and Saturation, Ion Selectivity, Multi-Ion Occupancy. (Learning UCSF Chimera Program) |
| 第 6 週 | Ion Permeations(II): Structural basis of K+ permeation through K+ channel pore (Learning UCSF Chimera Program) |
| 第 6 週 | Ion Permeations(II): Structural basis of K+ permeation through K+ channel pore (Learning UCSF Chimera Program) |
| 第 7 週 | Paper Discussion (Paper Discussion) |
| 第 7 週 | Paper Discussion (Paper Discussion) |
| 第 8 週 | Voltage-Dependent Mechanism (I): Voltage Dependence, Gating Charge, and Voltage Sensor (Learning HHSim computer program) |
| 第 8 週 | Voltage-Dependent Mechanism (I): Voltage Dependence, Gating Charge, and Voltage Sensor (Learning HHSim computer program) |
| 第 9 週 | Voltage-Dependent Mechanism (II): Voltage Dependence, Gating Charge, and Voltage Sensor (Hands-On Lab practice: Voltage-clamp & Patch-Clamp) |
| 第 9 週 | Voltage-Dependent Mechanism (II): Voltage Dependence, Gating Charge, and Voltage Sensor (Hands-On Lab practice: Voltage-clamp & Patch-Clamp) |
| 第 10 週 | Paper Discussion (Paper Discussion) |
| 第 10 週 | Paper Discussion (Paper Discussion) |
| 第 11 週 | Stochastic Behaviors of Single Ion Channels (Hands-On Lab practice: Voltage-clamp & Patch-Clamp) |
| 第 11 週 | Stochastic Behaviors of Single Ion Channels (Hands-On Lab practice: Voltage-clamp & Patch-Clamp) |
| 第 12 週 | Ion Channel pharmacology (Hands-On Lab practice: Voltage-clamp & Patch-Clamp) |
| 第 12 週 | Ion Channel pharmacology (Hands-On Lab practice: Voltage-clamp & Patch-Clamp) |
| 第 13 週 | Inactivation Mechanism (Paper Discussion) |
| 第 13 週 | Inactivation Mechanism (Paper Discussion) |
| 第 14 週 | Paper Discussion (Paper Discussion) |
| 第 14 週 | Paper Discussion (Paper Discussion) |
| 第 15 週 | Ion Channels and Diseases (Paper Discussion) |
| 第 15 週 | Ion Channels and Diseases (Paper Discussion) |
| 第 16 週 | Final Term Paper writing (Final Term Paper writing) |
| 第 16 週 | Final Term Paper writing (Final Term Paper writing) |
1. Hille, B. (2001) Ion Channels of Excitable Membranes, 3rd Ed. Sinauer Associates, Inc. Sunderland, MA, USA. 2. Handbook of Ion Channels, 2nd Ed. (2023)