基因操作及表現
Gene manipulation and expression
| 節 | 週四 |
|---|---|
5 13:20–14:10 | 基因操作及表現 YEC102 2 節連堂 |
6 14:20–15:10 |
* 根據陽明交大上課時間表所列
課程內容包括Fundamental techniques of gene manipulation, Plasmids, cosmids, and other vectors, Gel electrophoresis, Gene cloning strategies, Extraction, purification, and analysis of genomic DNA and mRNA from eukaryotic cells, Cloning in bacteria, yeast, and other fungi, PCR and DNA sequencing, Construction of genomic DNA and cDNA libraries, Analysis of gene expression, Mutagenesis and protein engineering, Gene transfer to animal cells, RNA interference, Gene targeting, CRISPR/Cas9 and targeted genome editing, Genetic manipulation of animals, Applications of recombinant DNA technology
使學生具有基因操作及表現相關的基本知識，以及瞭解此技術的重要性、應用性，這些課程將有助於學生的論文研讀及研究工作。
筆試（期中與期末考）、課中討論、分組討論、書面報告
| 週次 | 主題 |
|---|---|
| 第 1 週 | Introduction |
| 第 2 週 | Fundamental techniques of gene manipulation |
| 第 3 週 | Plasmids, cosmids, and other vectors |
| 第 4 週 | Gene cloning strategies |
| 第 5 週 | Extraction, purification, and analysis of genomic DNA and mRNA from eukaryotic cells |
| 第 6 週 | Intercollegiate activities |
| 第 7 週 | PCR and DNA sequencing |
| 第 8 週 | Mid-term exams |
| 第 9 週 | Construction of genomic DNA and cDNA libraries |
| 第 10 週 | Analysis of the transcriptome |
| 第 11 週 | Mutagenesis and protein engineering |
| 第 12 週 | Gene transfer to animal cells |
| 第 13 週 | Large-scale mutagenesis and interference |
| 第 14 週 | Applications of recombinant DNA technology |
| 第 15 週 | Application of genome editing |
| 第 16 週 | Final exams |
Principles of Gene Manipulation and Genomics: Principles and Applications of Recombinant DNA and Genomics; Richard M. Twyman and Sandy Primrose; Blackwell Pub., 8th ed., 2016