奈米載體於診斷與治療之進展與挑戰
Advances and Challenges of NanoCarrier Diagnostics and Therapeutics
| 節 | 週五 |
|---|---|
5 13:20–14:10 | 奈米載體於診斷與治療之進展與挑戰 YC101 4 節連堂 |
6 14:20–15:10 | |
7 15:30–16:20 | |
8 16:30–17:20 |
* 根據陽明交大上課時間表所列
Course abstract：We review the recent progress on diagnostics and therapeutics based on extracellular vesicles (EVs, lipoproteins), supermeres and exomeres. These nanocarriers from diseased cells are low in abundance (102 to 106 per ml of blood) and are extremely heterogeneous with different subsets carrying distinct biomarker cargoes. The ligands and receptors on their surface can also direct them to specific cells, thus enabling non-immunogenic drug delivery with long retention time. We examine the key issues that have so far prevented regulatory approval of the nanocarrier diagnostics and therapeutics, after nearly two decades of intense research. We also suggest possible research directions that may overcome these challenges, including some from my lab that are now ready to be commercialized.
| 週次 | 主題 |
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| 第 1 週 | |
| 第 2 週 | 3/6 Nanocarriers (NC) and Biogenesis: HDL, LDL, lEV, sEV, exomeres, supermeres, midbody remnants, ribonucleoproteins. NC Isolation and Purification: Ultracentrifugation, microfiltration, precipitation (Exoquick), tangential flow filtration, Asymmetric Flow Field Flow Filtration, Acoustic, NanoEx, Exodus. NC Biomarker Identification: Proteomics Mass Spect, NGS, NanoString 4 Dr. Hsueh-Chia Chang |
| 第 3 週 | 3/13 Key Metrics for NC Dx: Specificity, Sensitivity, Dynamic Range, Multiplexing, Assay Time NGS/PCR Based Diagnostics: Proximity Ligation Assay (Mercy Halo, Olink, Alamar), ddPCR Fluorescence Based EV Diagnostics: Exoview, Nanoflow, SIMOA, EV ELISA, Plasmonic Enhancement. Enzymatic Assays: Colocalization, digital nanoarray Charge-Based Sensors: Field-Effect Transistors, Ion Exchange Membrane (IEM) Size-Based Sensors: Surface Acoustic Wave, Immuno-Janus particle Integration for Point-of-Care Applications 4 Dr. Hsueh-Chia Chang |
| 第 4 週 | 3/20 NC Therapeutics : Nanomedicine, Don’t-Eat Me Tetraspanin (non-immunogenic), Targeting Ligand. LoadingTechnologies: Nanotransfection, Liposome, Transporter, Osmotic Preservation and DeliveryTechnologies: Spray Encapsulation, Inhalable Aerosol, Injectable Microgel Challenges: Heterogeneity, Retention, Quality Control 4 Dr. Hsueh-Chia Chang |
| 第 5 週 | 3/27 Beachhead Markets and Future Pathways Unregulated Markets: Cosmetics, Pet Care, Agricultural/GMO Research Markets: Biomarker and Therapeutic Target Discovery High-Throughput Drug Testing with Organoids Low-Hurdle Medical Markets: Psychological Disorder Neurodegenerative Diseases Holy Grail Market: Therapy Management AI-Assisted Large-Population Pan-Disease Screening Student Presentation 4 Dr. Hsueh-Chia Chang |
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