光譜與分子量測學

Biospectroscopy and Detection

學期

114-2

學分

3 學分

當期課號

410034

永久課號

BTBT10064

開課單位

生物科技學系

授課教師

高雅婷

校區

光復

類別

必修

上課時間表

節	週四
2 09:00–09:50	光譜與分子量測學 SC109 3 節連堂
3 10:10–11:00
4 11:10–12:00

* 根據陽明交大上課時間表所列

概述

課程概述與目標：詳細概論光學光譜理論‚並介紹如何將這些理論運用於現代分子和細胞生物物理和生物化學◦ This course will provide fundamental concepts on optical spectroscopy, which is used to study the structure and function of biological macromolecules. This course aims to help students develop a physical understanding of daily-used instruments in biophysical and biochemistry laboratories.

先修科目

先修科目或先備能力：基礎物理、基礎化學 (General physics and chemistry)

評分方式

5 homeworks (30%) one midterm and one final exam (70%, 35% each exam)

課程大綱

Basic concepts in quantum chemistry
Light and Matters
The electronic states
Microscopy
The vibrational states
Homework recitations

週次計畫

週次	主題
第 1 週	 Course Introdunction  From Classical to quantum mechanics
第 2 週	 The Schrödinger equation  The Quantum Mechanical Postulates
第 3 週	 The particle in a box and the real world  The Harmonic oscillator
第 4 週	 Rigid rotator  The hydrogen atom  Many electron atoms  LCAO-MO
第 5 週	 Interaction of radiation with light  Einstein A and B coefficients  Absorption and emission of Radiation (Jabloński diagram)  The Frank-Condon Principle Absorption spectroscopy
第 6 週	 UV-VIS Absorption spectroscopy  Transition dipole moment  Light-dependent Schrödinger equation  Symmetry-allow and Symmetry-forbidden  Beer-Lambert Law (empirical results and limitation)
第 7 週	 UV-VIS Absorption spectroscopy (Application)  Fundament instruments  CD and ORD (protein secondary structures)
第 8 週	 Fluorescence spectroscopy I  Introduction to fluorescence  Characteristics of fluorescence emission  Fluorophores: Dye, biomolecules, and fluorescent protein
第 9 週	 Midterm
第 10 週	 Fluorescence spectroscopy II (Applicataion)  Fluorescence lifetime  Absorption spectroscopy  Förster resonance energy transfer (FRET)  Fluorescence sensing (Immunoassays….)
第 11 週	 Fluorescence spectroscopy III (detection and imaging)  Instrumentation  Time-resolved measurements  Fluorescence microscopy (FRAP, FLIM…)
第 12 週	 Microscopy I  Basic light microscopy  Confocal microscopy  Multiphoton fluorescence microscopy
第 13 週	 Microscopy II  Optical sectioning microscopy (TIRFM, LSFM…..)  Superresolution (STED, SSIM, PALM, STORM….)
第 14 週	 Principles of infrared spectroscopy  Molecular symmetry  Vibrational spectra of biopolymers
第 15 週	 Spontaneous Raman  Resonance Raman  Raman spectroscopy and imaging  Microscopy (CARS, SRS)
第 16 週	Final exam

教科書

Peter Atkins, Physcial chemistry, 121th Ed. 以下為參考書目: • Joseph. R. Lakowicz, Principles of fluorescence spectroscopy, 3rd Ed., 2006 • Thomas Engel, Quantum chemistry and spectroscopy, 3rd Ed., 2012