實驗分子生物

Time: 2:20 pm ~ 5:00 pm on Tuesdays Place: R208 of the Institute of Biological Chemistry, Academia Sinica Credit: 3 credits Perspective: A holy grail in biology is to modulate the functions of bio-molecules. For example, one wants to design small compounds to perturb the form and activities of bio-molecules for disease intervention (e.g., inhibition of protein aggregation for treating neurodegenerative diseases). These pursuits will not be possible without proper knowledge (e.g., structure, function, composition, interactome, localization) of the bio-molecules at hand. This course will give you a first-hand look at the many biophysical techniques that allow you to characterize your biomolecule of interest through lectures, facility visits, hands-on sessions, and designed presentations. Topic A: Spectroscopy and Solution Biophysics This module is to lecture on general spectroscopy techniques for peptides, proteins, and protein-protein interactions. The techniques to be covered include fluorescence, circular dichroism, infrared spectroscopy, fluorescence life-time imagiang ...etc. Topics on protein misfolding and therapeutic strategies against protein misfolding will be introduced. A1: Principle of spectroscopy and protein misfolding (peptide synthesis, CD, fluorescence, and infrared spectroscopy) A2: Theraputic strategies against protein misfolding (nanoparticle preparation, fluorescence life-time imaging, Fluorescence recovery after photobleaching) A3: Hands-on experiments (peptide synthesis, CD, fluorescence imaging) A4: Student Presentation (present related studies on protein misfolding or therapeutic strategy against protein misfolding diseases) Topic B: Imaging This module will take you into the world of bio-imaging. We will help develop your intuition on bio-imaging, show you what microscopes there are on campus, and go through various imaging tricks that can help advance your research. B1: Fun facts of imaging: distribution, resolution, and contrast mechanisms B2: The many types of fluorescence microscopes (Hands-on session: using a confocal microscope) B3: Inside image quantification (Hands-on session: playing with ImageJ. //personal laptop required) B4: Case study: spatial genomics- transcriptomics through imaging (short student presentations) Topic C: Crystallography This module is to lecture on the techniques for single-crystal X-ray diffraction and its applications and limitations. We will also use lysozyme as a model protein to go through the steps of protein crystallization, X-ray data collection and analysis C1: Protein crystallization Hands-on training: Hanging-drop protein crystallization C2: Why X-ray crystallography? Hands-on training: Crystal mounting C3: Principle of X-ray diffraction Hands-on training: X-ray diffraction C4: Data analysis Hands-on training: Data analysis Topic D: Mass Spec The goal of this module is to introduce the principle of mass spectrometry (MS) and the progress made in technological development. The application of MS for a range of biology/pharmaceutics studies and its emerging utility in investigating protein structures and dynamics will be further discussed. D1: History and basis of mass spectrometry D2: The application of mass spectrometry in “mocis” studies Hands-on session: Instrumentation and data analysis) D3: State-of-the-art mass specteometry in investigating structural and dynamical property of protein molecules D4: Student presentation/Special talk

Attendance: Unexplained absence: -3 of final grade Late: -1 of the final grade Absence without supporting document: -1 of final grade *Students who need to take leave should contact the course TA or the program secretary, Ms. Vicki Huang (vicki0315@gate.sinica.edu.tw) with proper reason and proof before the lecture starts. If not, will be considered as unexplained absence.

Grading scheme: 25% for each module (Details will be announced at each module)  Topic A: Spectroscopy and Solution Biophysics  Topic B: Imaging  Topic C: Crystallography  Topic D: Mass Spec